普浩宇,亐开兴,杨贵伟,程美玲,黄必志,王蓉,任珂青,尹革芬

• 1:云南农业大学动物医学学院
• 2:云南省草地动物科学研究院

摘要(Abstract)：

本研究利用MDBK细胞分离牛病毒性腹泻病毒(bovine viral diarrhea virus, BVDV),通过IFA、qRT-PCR和ELISA方法鉴定,从云南分离获得了1株基因2型BVDV,命名为YNJG。采用IFA,按Reed-Muench法计算出YNJG分离株的滴度为10~(-4.4 )TCID_(50)/100μL。根据其5′UTR序列进行基因分型,确定YNJG属于BVDV-2毒株。全基因序列测序结果显示,YNJG毒株全长为12 288个核苷酸,编码3 898个氨基酸,GenBank登录号为MW168422,与美国BVDV分离株9231核苷酸同源性最高,为95.4%,该毒株不能导致细胞病变,属于非致细胞病变型毒株。毒力分析显示,该毒株5′UTR第216位和275位核苷酸分别为C和T,且1 142位氨基酸处无氨基酸的插入,属于低毒力毒株。

关键词(KeyWords)： 牛病毒性腹泻病毒基因2型;YNJG分离株;5′UTR;遗传进化分析

Abstract:

Keywords:

基金项目(Foundation): 云南省高校科技创新团队支持计划资助项目(IRTSTYN);; 云南省后备人才培养计划基金资助项目(2018HB045);; 云南省重点研发专项基金资助项目(2019ZG011)

作者(Author): 普浩宇,亐开兴,杨贵伟,程美玲,黄必志,王蓉,任珂青,尹革芬

DOI: 10.16303/j.cnki.1005-4545.2021.10.06

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